《植物生理学报》 2012, 48(6): 597-604

拟南芥漆酶基因AtLAC2调控植物生长发育的研究

张盛春, 张玉平, 王小菁*

华南师范大学生命科学学院广东省植物发育生物工程重点实验室, 广州510631

通信作者：王小菁；E-mail: wangxj@scnu.edu.cn；Tel: 020-85216417

摘　要：

拟南芥漆酶基因家族有17个成员, 但其功能还不清楚。本文采用过量表达的方法初步分析了拟南芥漆酶基因At- LAC2的功能。GUS染色显示AtLAC2在拟南芥不同生长发育时期的多个组织和器官中都有较强的表达, 且在叶片和茎尖中 的表达最强。AtLAC2的过量表达植株开花时间推迟, 花序轴分枝变多, 叶片变小。以上结果表明AtLAC2基因在调控植物 生长发育中具有重要作用。

关键词：拟南芥; AtLAC2; 分枝; 开花; 漆酶

收稿：2012-03-15   修定：2012-05-25

资助：国家自然科学基金(30900789)和国家自然科学基金委员 会-广东省人民政府联合基金(U0631001)。

Arabidopsis thaliana Laccase Gene AtLAC2 Regulates Plant Growth and Development

ZHANG Sheng-Chun, ZHANG Yu-Ping, WANG Xiao-Jing*

Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China

Corresponding author: WANG Xiao-Jing; E-mail: wangxj@scnu.edu.cn; Tel: 020-85216417

Abstract:

There are 17 genes in Arabidopsis laccase gene family. However, their functions have not been clearly demonstrated. The present study analyzed the function of the AtLAC2 gene by over-expressing it in the wildtype plants. The GUS staining results showed that AtLAC2 was strongly expressed in various organs and tissues during different developmental periods, especially in the leaf and shoot apex. AtLAC2 over-expressing plants showed delayed flowering time, increased primary branches, and decreased leaf size. These results suggest that AtLAC2 may play important roles in regulating plant growth and development.

Key words: Arabidopsis thaliana; AtLAC2; branches; flowering; laccase